Title Characterization of virulent bacteriophage specific for < I > Escherichia coli < / I > O 157 : H 7 and analysis of it ’ s cellular receptor and two tail fiber genes
نویسندگان
چکیده
Article / Book Information Title Characterization of virulent bacteriophage specific for Escherichia coliO157:H7 and analysis of it’s cellular receptor and two tail fiber genes Author M. Morita, Y. Tanji, K. Mizoguchi, T. Akitsu, N. Kijima, H. Unno Journal/Book name FEMS Microbiol. Letter, Vol. 211, No. , pp. 77-83 Issue date 2002, URL http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1574-6968 DOI http://dx.doi.org/10.1111/j.1574-6968.2002.tb11206.x
منابع مشابه
Characterization of a virulent bacteriophage speci¢c for Escherichia coli O157:H7 and analysis of its cellular receptor and two tail ¢ber genes
A virulent phage, named PP01, specific for Escherichia coli O157:H7 was isolated from swine stool sample. The phage concentration in a swine stool, estimated by plaque assay on E. coli O157:H7 EDL933, was 4.2U107 plaque-forming units per g sample. PP01 infects strains of E. coli O157:H7 but does not infect E. coli strains of other O-serogroups and K-12 strains. Infection of an E. coli O157:H7 c...
متن کاملDistribution of virulence associated genes in isolated Escherichia coli from avian colibacillosis
BACKGROUND: Colibacillosis is one of the most prevalentdiseases in the world that causes multimillion-dollar annuallosses. OBJECTIVES: In order to evaluate molecular epidemiologyof some virulence associated factors in Escherichia coli,isolated from poultry, the presence of iut A, iss, hly F, omp T, iroN, afa, sfa (S)and pap G (II) were investigated by multiplex PCRassay. METHODS: Two hundred th...
متن کاملBacteriophage K1-5 encodes two different tail fiber proteins, allowing it to infect and replicate on both K1 and K5 strains of Escherichia coli.
A virulent double-stranded DNA bacteriophage, Phi K1-5, has been isolated and found to be capable of infecting Escherichia coli strains that possess either the K1 or the K5 polysaccharide capsule. Electron micrographs show that the virion consists of a small icosohedral head with short tail spikes, similar to members of the Podoviridae family. DNA sequence analysis of the region encoding the ta...
متن کاملPURIFICATION AND CHARACTERIZATION OF THE CLONED HUMAN GM-CSF GENE EXPRESSED IN ESCHERICHIA COLI
The human granulocyte-macrophage colony stimulation factor (hGM-CSF) gene was cloned in the pET 23a( +) expression vector under the control of strong bacteriophage T7 transcription and translation signals. The hGM-CSF gene was transferred into E. coli strainBL21 (DE3)pLysS andIPTG was used for induction of GM-CSF gene. Production of the target protein was obtained as revealed by ELISA and ...
متن کاملIn vitro assessment of pathogenicity and virulence encoding gene profiles of avian pathogenic Escherichia coli strains associated with colibacillosis in chickens
Background: Avian pathogenic Escherichia coli (APEC) strains have been associated with various disease conditions in avian species due to virulence attributes associated with the organism. Aims: This study was carried out to determine the in vitro pathogenic characteristics and virulence encoding genes found in E. coli strains associated with colibacillosis in chickens. Methods: Fifty-two stock...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2017